| 质粒名称: | pSec61b-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000762 |
| 产品用途: | Expresses Sec61b-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Sec61b, mScarlet3-H |
| 物种来源: | H.sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
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pSec61b-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSec61b-mScarlet3-H (aka mYongHong)载体质粒基本信息
pSec61b-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pSec61b-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pSec61b-mScarlet3-H (aka mYongHong)质粒载体简介
pSec61b-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pSec61b-mScarlet3-H (aka mYongHong) 4924 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_762_20241014-BAN4_E04_E04_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4924)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4924
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 95..683
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 858..1161
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1162..1365
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1394..2080
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
CDS 2096..2386
/codon_start=1
/gene="SEC61B"
/product="human Sec61-beta subunit of the translocon in the
endoplasmic reticulum"
/label=Sec61-beta
/translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
polyA_signal 2509..2630
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2637..3092)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3119..3223
/gene="bla"
/label=AmpR promoter
promoter 3225..3582
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3433..3568
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3617..4411
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4643..4690
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
61 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
781 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
841 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
901 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
961 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
1021 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
1081 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
1141 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
1201 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
1261 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
1321 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
1381 cagatccgct agcatggata gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt
1441 gcacatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
1501 cccctacgag ggcacccaga ccgccaagct gagggtgacc aagggtggcc ccctgccctt
1561 ctcctgggac atcctgtccc ctcagttcat gtacggctcc agggccttca cgaagcaccc
1621 cgccgacatc cccgactact ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt
1681 gatgaacttc gaggacggcg gcgccgtgtc cgtggcccag gacacctccc tggaggacgg
1741 caccctgatc tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat
1801 gcagaagaag acaatgggct gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt
1861 gctgaagggc gacattaagc atgccctgcg cctgaaggac ggcggccggt acctggcgga
1921 cttcaagacc acctacaggg ccaagaagcc cgtgcagatg cccggcgcct tcaacatcga
1981 ccgcaagttg gacatcacat cccacaacga ggactacacc gtggtggaac agtacgaacg
2041 ctccgtggcc cgccactcca ccggcggctc cggtggctcc ccaccggtcg ccaccatgcc
2101 tggtccgacc cccagtggca ctaacgtggg atcctcaggg cgctctccca gcaaagcagt
2161 ggccgcccgg gcggcgggat ccactgtccg gcagaggaaa aatgccagct gtgggacaag
2221 gagtgcaggc cgcacaacct cggcaggcac cggggggatg tggcgattct acacagaaga
2281 ttcacctggg ctcaaagttg gccctgttcc agtattggtt atgagtcttc tgttcatcgc
2341 ttctgtattt atgttgcaca tttggggcaa gtacactcgt tcgtagagcg gccgcgactc
2401 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
2461 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
2521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
2581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
2641 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
2701 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
2761 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
2821 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
2881 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
2941 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
3001 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
3061 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
3121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
3181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
3241 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
3301 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
3361 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3421 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3481 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
3541 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
3601 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
3661 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
3721 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
3781 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
3841 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
3901 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
3961 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
4021 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
4081 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
4141 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
4201 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
4261 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
4321 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
4381 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
4441 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
4501 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
4561 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
4621 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
4681 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
4741 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
4801 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
4861 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
4921 gtga
//
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